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. Author manuscript; available in PMC: 2014 May 2.
Published in final edited form as: Cancer Lett. 2010 Jul 23;298(2):159–166. doi: 10.1016/j.canlet.2010.06.014

Figure 1. 1,25(OH)2D regulation of HIF-1α protein and mRNA levels.

Figure 1

MCF10A and MCF10A-ras cells were treated with either vehicle (open bars) for 12hrs or with 1,25(OH)2D (10 nM) (black bars) for indicated times. Following harvest, lysates were used for either Western blot analysis or mRNA isolation as described. A representative immunoblot of HIF-1α and actin protein level is shown in panel A. The quantification of the immunoblots is shown in panel B (top left) for MCF10A cells and MCF10A-ras cells (panel B, top right). Values are expressed as HIF-1α protein/actin protein for each sample relative to vehicle. The mRNA abundance of HIF-1α, determined by RT-PCR is shown in panel B (bottom right) for MCF10A cells and MCF10A-ras cells (panel B, bottom left). Values are expressed as HIF-1α mRNA/18S mRNA. Results are expressed as mean ± SEM. (n ≥ 5). Bars with common letter superscripts are not significantly different (p< 0.05). Data are representative of three independent experiments.