Table 1. List of primers used for real-time RT-PCR.
Target gene | Primer pairs (5′-3′)a | Amplicon size (bp) |
---|---|---|
18s | (F) TCTTTCTTGATTTTGTGGGTGG (R) TCGATAGTCCCTCTAAGAAGTG | 150 |
ERG1 | (F) TTAGAATCATGCCAAACC (R) CCAACTGTCATACCACCC | 127 |
ERG2 | (F) TAATAATGCTGGTGGTGC (R) CAGGATAAGCTGCTCTTT | 167 |
ERG3 | (F) GTCTAATGACCCAGTTGT (R) TCTTCTTCTGCCTTTGCA | 162 |
ERG4 | (F) TATACGCCAATGCTTGTG (R) AGTAACTGAATGGAACCC | 120 |
ERG5 | (F) AGATACCGTCCACCAGTC (R) TGCAAAGCAGGATACAAT | 119 |
ERG6 | (F) GCTACCGTTCATGCTCCA (R) CCATCACCGACTTCAATA | 164 |
ERG7 | (F) GCTTGGGCTTTGATAGGG (R) TCCACTCACCAGTCGGTA | 106 |
ERG10 | (F) TGCCTTGGGTCATCCTCT (R) CCGTTACAAACACCAGCA | 108 |
ERG11 | (F) GAATCCCTGAAACCAAT (R) AGCAGCAGTATCCCATC | 131 |
ERG13 | (F) TGGAACACGCTTACGATT (R) CACATGGAAGGCACTGAA | 191 |
ERG24 | (F) GGTGACTTAGCGTGGGT (R) GCTGAGCGGAAGATGTA | 143 |
a F, forward; R, reverse.