FIGURE 3.

Effect of SRA on the accumulation of pulmonary leukocytes. Lung leukocytes were isolated from infected SRA^+/+ and SRA^−/− mice at 3 wpi, Ab stained, and analyzed using flow cytometry as per Materials and Methods. In brief, leukocytes were identified as CD45⁺ cells. Next, additional gates were set up to identify lymphocytes (CD3⁺ and CD19⁺), neutrophils (Ly6G⁺/CD11b⁺), eosinophils (SSC^high/CD11c^int), Ly6C⁺ monocytes (CD11c⁻/Ly6C⁺/CD11b⁺), macrophages (autofluorescence⁺/CD11c⁺), and CD11b⁺ DCs (autofluorescence⁻/CD11c⁺/CD11b⁺). The total number of CD45⁺ leukocytes was determined by multiplying the frequency of CD45⁺ by the total number cells. Total numbers of eosinophils (A), neutrophils (B), monocyte (C), macrophages (D), CD11b⁺ DCs (E), and lymphocytes (F) were determined by multiplying the frequency of each subset by the total number of CD45⁺ leukocytes at 3 wpi. Data represent mean ± SEM pooled from one separate experiments; n ≥ 6 for each of the analyzed parameters; *p <0.05 in comparison between SRA^+/+ and SRA^−/− mice. NS, No significant difference between SRA^+/+ and SRA^−/− mice.