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. Author manuscript; available in PMC: 2014 May 2.
Published in final edited form as: J Immunol. 2013 Jun 3;191(1):238–248. doi: 10.4049/jimmunol.1203435

FIGURE 6.

FIGURE 6

Effect of SRA on fungicidal activity of macrophages. BMDMs were generated from uninfected SRA+/+ or SRA−/− mice as per Materials and Methods. After harvest, BMDMs were seeded on 24-well plate with 1 × 106 cells/well, then infected with 1 × 105 Abopsonized C. neoformans lac1Δ, and incubated for 24 h at 37°C and 5% CO2 in media alone or with recombinant mouse cytokines: IL-4 (20 ng/ml), IFN-γ (100 ng/ml), or a combination of IFN-γ (20 ng/ml) and TNF-α (20 ng/ml). After the incubation, the yeast were collected by lysing the macrophages and enumerated by plating 10-fold serial dilutions for each sample onto Sabouraud dextrose agar plates. Colonies were counted after 48 h. CFU values in individual treatment groups were compared with culture wells without macrophages and expressed as percent of C. neoformans–growth inhibition. Data represent mean ± SEM pooled from several separate experiments; n ≥ 3 for each of the analyzed parameters. NS, No significant difference between SRA+/+ and SRA−/− mice.