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. Author manuscript; available in PMC: 2014 May 2.
Published in final edited form as: J Immunol. 2013 Jun 3;191(1):238–248. doi: 10.4049/jimmunol.1203435

FIGURE 8.

FIGURE 8

Effect of SRA on the immune response in the lung and LALNs at 1 wpi. The BAL fluid and LALNs were collected from uninfected and infected SRA+/+ and SRA−/− mice. The BAL fluid was analyzed by ELISA to assess cytokines expression (A). Total RNA extracted from the cells of LALN was converted to cDNA and analyzed by real-time RT-PCR for the production of cytokine and transcript regulators of CD4+ T cells (B). Note a robust increase of IFN-γ and a significant deficiency in the expression of IL-4 and IL-13, in the BAL of infected SRA−/− mice, compared with infected SRA+/+ mice. And also note a significant induction of IFN-γ, IL-12a, and Th1 transcript regulator T-bet in the LALNs of infected SRA−/− mice. Data were pooled from two to three separate matched experiments; n ≥ 6 for each of the analyzed parameters. *p <0.05 in comparison between SRA+/+ and SRA−/− mice.