Table 3.
Spa -typing of S. aureus strains from a single individual AE with two sets of primers: standard primers 1095 F/1517R and novel primers spaT3-F/1517R
|
Time points, months |
DNA prep (mixed boilate) |
12 single colony picks
2
|
|
|---|---|---|---|
| 1095 F/1517R | spaT3-F/1517R | spaT3-F/1517R | |
| AE-0 |
t230 |
MST1 |
t230/t012 |
| AE-1 |
non-typable |
t012 |
t012 |
| AE-2 |
t230 |
MST |
t230/t012 |
| AE-4 |
t230 |
MST |
t230 |
| AE-6 |
t230 |
MST |
t230/t528 |
| AE-8 |
t008 |
MST |
t008/t012/t571 |
| AE-10 |
t230 |
MST |
t230 |
| AE-12 |
t230 |
MST |
t230 |
| AE-14 | t230 | MST | t230 |
1mixed sequence traces; 2spa-types in bold have delE and could not be typed with standard primers; spa-repeats:
t230: 08-16-02-16-34.
t571: 08-16-02-25-02-25-34-25.
t008: 11-19-12-21-17-34-24-34-22-25.
t012: 15-12-16-02-16-02-25-17-24-24.
t528: 04.