Fig 2.

Cell cycle analysis of carnosine-treated cell lines. (A) The SGC-7901 cells were treated with carnosine (50 and 100 mM) and rapamycin (20 nM) for 24 h. Then, the cell cycle distribution was measured using flow cytometry. The experiments were performed in triplicate. (B) Cell cycle distribution was analysed by flow cytometry in MKN45 cells 24 h after they were treated with carnosine (50 and 100 mM) and rapamycin (20 nM). The percentage of cells in each population was shown as the mean ± SD from three independent experiments. (C) Western blot analysis of the expression of p21, Cyclin A and Cyclin E in SGC-7901 and MKN45 cells after carnosine and rapamycin treatment for 48 h. (D) The intensity of bands of western blot were quantified by scanning densitometry. Data are means ± SD. *P<0.05, **P<0.01.