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Catalytic activities of the indicated SHP2 catalytic domain (221–524) GST fusion proteins were measured using the Malachite Green assay in the presence of different concentrations of PTP-1B peptide R-R-L-I-E-D-A-E-pY-A-A-R-G. Km and kcat calculated by: 1/V = (Km/Vmax) / [pY] + 1/ Vmax
| k cat (s −1 ) | K m (mM) | kcat / Km (s−1 mM−1) | |
|---|---|---|---|
| WT |
9.26 |
1.31 |
7.07 |
| N308D |
12.82 |
1.92 |
6.68 |
| Y279C |
0.12 |
9.14 |
0.01 |
| Q506P | 0.61 | 2.83 | 0.22 |
