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. 2014 Mar 13;14:14. doi: 10.1186/1471-213X-14-14

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Copyright © 2014 Glebov et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Pharmacological analysis of 5-HT receptors involved in retardation and acceleration of H. trivolvis development. (A-C). Development tempo expressed as changes in the length of embryos is modulated upon treatment with different 5-HTR agonists in a dose-dependent manner. Treatment with RS67333 (A) and 8-OH-DPAT (B) results in retardation, while treatment with 5-CT (C) – in acceleration effects. (D) Effect of combined application of agonists and antagonists on the embryos length after 84 h of incubation. Data were normalized to the control and shown as mean ± s.d. (***, p <0.001). (E) Effect of forskolin treatment on the embryos length after 84 h of incubation. Normalized values represent means ± s.d.; (***, p <0.001). (F) Effect of db-cAMP treatment on the embryos length after 84 h of incubation. Normalized values represent means ± s.d.; (***, p <0.001).