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. 2014 Mar 13;14:14. doi: 10.1186/1471-213X-14-14

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Copyright © 2014 Glebov et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of the 5-HT₁and 5-HT₇receptors is differently regulated during H. trivolvis development. Relative changes in the amount of mRNA encoding for the 5-HT₁ and 5-HT₇ receptors were determined at different developmental stages, including trochophore (st 19), shortly before metamorphosis (st 23) and in the middle of metamorphosis (st 25) using real time quantitative RT-PCR and ΔΔCt method. (A) The 5-HT₁R is transiently unregulated at stage 23 only. (B) Relative expression of 5-НТ₇R gradually down regulated by the metamorphic stage (st 25).