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. 2014 Mar 13;14:14. doi: 10.1186/1471-213X-14-14

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Copyright © 2014 Glebov et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effects of different 5-HT receptor agonists on cell proliferation during H. trivolvis development. (A) Representative confocal images of the whole-mount H. trivolvis embryos co-stained with DAPI (blue) and the antibody against phospho-H3S28 (red) after incubation with RS67333, 8-OH-DPAT and 5-CT (5 μM each). Staining with anti-phospho-H3S28 antibody allows visualization of dividing cells. Scale bars are 20 μM for the stage 19, and 50 μM for the stages 23 and 25. (B) The relative number of dividing cells at stages 19, 23 and 25 after 10 h of incubation with RS67333, 8-OH-DPAT and 5-CT. Each value represents the mean ± s.e.m. (n = 10). A statistically significant difference between values is noted (** p <0.01, *** p <0.001).