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. 2014 Mar 13;2:29. doi: 10.1186/2051-5960-2-29

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Copyright © 2014 Bétemps et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of disease-associatedα-synuclein (αS^D) in different neuroanatomical regions of M83 mice after stereotactic inoculations in the hippocampus (A-C) or cerebellum (D-F) A, D. Detection of αS on sagittal brain sections by immunohistochemistry using PSer129 antibody. (Scale bar, 1 mm. Insets are high magnifications of different areas). Arrows indicate the approximate sites of the stereotactic inoculations. B, E. Detection of insoluble pSer129 αS by Western blot in selected neuro-anatomical regions. β-actin was used as loading control. C, F. ELISA results with LB509 and PSer129 antibody in selected neuro-anatomical regions. Hi: hippocampus (Hi), C: contro-lateral side, I: inoculated side, Cb: cerebellum, BS: brainstem, SC: spinal cord.