Figure 4.

Breast cancer cell line panel study. A panel consisting of 10 human breast cancer cell lines are individually deposited on the hybrid platform. The jamming coefficient of EphA2 is a ratio of the radial profiles measured for cells that were deposited on bilayer only side to the radial profiles of cells on the hybrid side (see supporting information). The cells with the highest normalized jamming coefficient exhibit the most frustrated EphA2 transport. For all cell lines, on the glass side displaying only mobile ephrin-A1, EphA2 is centrally transported. Differential behavior among the cell lines was observed on the nanodot side, on which ephrin-A1 is presented in the hybrid configuration. The animal studies of tumorigenicity and metastasis which correlate with the EphA2 jamming coefficients, the invasion potential values, the EphA2 protein expression levels, and EphA2 mRNA levels were taken from published sources.^24-31 The cell line SKBR3 was found to be tumorigenic in one report (*).³² A total of 200 cells were selected and analyzed. Scale bars are 10 microns.