Table 1.
Purification of the recombinant PelN from E. coli BL21 (DE3) *
| Fraction | Activity ** (kU) | Protein (mg) | Specific activity (U mg −1 ) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Crude extract of supernatant |
74.2 ± 0.6 |
109.8 ± 0.9 |
675.8 ± 55.9 |
1 |
100 |
| Ammonium sulphate precipitation |
46.6 ± 6.2 |
39.9 ± 3.9 |
1,166.5 ± 59.8 |
1.73 |
62.75 |
| UNOsphere S column | 21.5 ± 0.1 | 10.5 ± 0.3 | 2,060.0 ± 71.1 | 3.05 | 29.02 |
*Data represent means ± SD (n = 4) relative to the reference samples;
**The activity was measured on PGA under the standard conditions.