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. 2014 Mar 10;14:18. doi: 10.1186/1472-6750-14-18

Table 2.

Effects of different chemicals on the activity of the purified recombinant PelN

Chemicals Relative activity (%)*
Concentration (mM)
 0.5
 1.0
None
 100 ± 1
 100 ± 1
Ca2+
 729 ± 38
 437 ± 1
Mg2+
 125 ± 11
 99 ± 3
Mn2+
 75 ± 3
 57 ± 5
Cu2+
 79 ± 3
 104 ± 11
Zn2+
 116 ± 8
 106 ± 7
Fe2+
 82 ± 7
 57 ± 3
Ba2+
 12 ± 3
 3 ± 0
K+
 119 ± 8
 100 ± 4
EDTA
 nd
 nd
Triton X-100
 102 ± 9
 100 ± 8
Tween-20 98 ± 2 116 ± 11

nd: no detectable activity.

*The activity under the standard conditions without any chemicals was used as the reference (100%), and data represent means ± SD (n = 4) relative to the untreated reference samples;