Table 3.
Effects of different chemicals on the activity of the purified recombinant PelN*
|
Substrate |
Relative activity ** |
K
m
|
V
max
|
k
cat
|
k
cat
/
K
m
|
|
|---|---|---|---|---|---|---|
| (%) | (g L −1 ) | (mol mg−1 min−1) | (s −1 ) | (L s−1 g−1) | ||
| PGA |
100.0 ± 4.2 |
3.48 ± 0.35 |
4.12 ± 0.77 |
3.29 × 106 |
9.45 × 105 |
|
| Pectin |
20–34% |
100.9 ± 6.1 |
15.06 ± 1.24 |
15.16 ± 0.31 |
1.21 × 107 |
8.03 × 105 |
| 55–70% | 74.3 ± 2.6 | 3.32 ± 0.07 | 1.15 ± 0.09 | 0.92 × 106 | 2.77 × 105 | |
*Data represent means ± SD (n = 3) relative to the reference samples;
**The specific activity of PelN on PGA under the standard conditions was used as the reference (100%).