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. 2014 Feb 24;15(1):153. doi: 10.1186/1471-2164-15-153

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© Guimaraes et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Near-isogenic lines introgressed with qALT6 , using Cateto Al237 and L53 as the donor and recurrent parents, respectively. A) Aluminum tolerance measured as relative seminal root growth after five days under {39} μM Al³⁺. B) ZmMATE1 relative expression evaluated in root tips after 6 hours of treatment with {39} μM Al³⁺ (dark gray) and under control condition (light gray). Expression of L53 under controlled condition (−Al) was used as calibrator. C) ZmMATE1 copy-number estimated based on qPCR. Error bars indicate standard deviation.