Table 3.
Linear range
| Gene Assay | Linear Range (ng/well) | Quadratic p-value | Cubic p-value | Maximum Absolute Deviation from Linearity |
|---|---|---|---|---|
| ARF1 |
2-5.3 to 27.7 |
<.0001 |
0.1191 |
0.37 |
| ATP5E |
2-3.3 to 27.7 |
<.0001 |
0.0418 |
0.82 |
| AZGP1 |
2-3.3 to 27.7 |
0.0006 |
0.1143 |
0.46 |
| BGN |
2-5.3 to 27.7 |
0.0030 |
0.4064 |
0.57 |
| CLTC |
2-5.3 to 27.7 |
0.0145 |
0.2198 |
0.28 |
| COL1A1 |
2-5.3 to 27.7 |
0.2406 |
0.0268 |
0.31 |
| FAM13C1 |
2-3.3 to 27.7 |
0.9180 |
0.0026 |
0.81 |
| FLNC |
2-1.3 to 27.7 |
0.0002 |
0.0001 |
0.17 |
| GPS1 |
2-3.3 to 27.7 |
0.3704 |
<.0001 |
0.40 |
| GSN |
2-2.3 to 27.7 |
<.0001 |
0.8081 |
0.57 |
| GSTM2 |
2-1.3 to 27.7 |
0.2461 |
0.0009 |
0.23 |
| KLK2 |
2-5.3 to 27.7 |
0.3600 |
0.0008 |
0.16 |
| PGK1 |
2-3.3 to 27.7 |
0.0224 |
0.5250 |
0.30 |
| SFRP4 |
2-2.3 to 27.7 |
0.5033 |
0.1019 |
N/A |
| SRD5A2 |
20.7 to 210.7 |
0.0002 |
0.1016 |
0.34 |
| TPM2 |
2-5.3 to 27.7 |
<.0001 |
0.2190 |
0.43 |
| TPX2 | 20.7 to 210.7 | 0.5396 | 0.5799 | N/A |
N/A: No statistically significant departure from linearity.
Estimated linear ranges, associated p-values for quadratic or cubic regression models and maximum deviations from linearity for each of the 17 genes. The results were calculated using the data obtained from the 15-point dilution series.