Table 7.
Performance of the RT-PCR positive control
| Gene Assay | Mean | Median | SD |
|---|---|---|---|
| ARF1 |
22.7 |
22.7 |
0.20 |
| ATP5E |
24.3 |
24.2 |
0.33 |
| AZGP1 |
24.6 |
24.6 |
0.23 |
| BGN |
23.7 |
23.7 |
0.22 |
| CLTC |
24.0 |
24.0 |
0.19 |
| COL1A1 |
22.4 |
22.5 |
0.25 |
| FAM13C1 |
25.8 |
25.8 |
0.23 |
| FLNC |
25.3 |
25.3 |
0.24 |
| GPS1 |
25.3 |
25.3 |
0.20 |
| GSN |
25.9 |
25.9 |
0.29 |
| GSTM2 |
26.7 |
26.7 |
0.21 |
| KLK2 |
19.4 |
19.4 |
0.20 |
| PGK1 |
26.3 |
26.3 |
0.22 |
| SFRP4 |
26.7 |
26.7 |
0.23 |
| SRD5A2 |
28.1 |
28.0 |
0.32 |
| TPM2 |
22.6 |
22.6 |
0.21 |
| TPX2 | 29.6 | 29.6 | 0.25 |
Summary statistics, including standard deviations for the RT-PCR positive control performance during analytical and clinical validation were calculated on the non-normalized scale. The plates were assembled using multiple PCR and liquid handling robots and by multiple operators, using multiple lots of critical reagents spanning a period of 20 weeks (N=54 individual Cp measurements).