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. 2014 Mar 1;15(1):170. doi: 10.1186/1471-2164-15-170

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© Bottacini et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sorbitol gene cluster in B. breve and insertion mutant growth curve. a) Locus map showing the comparative analysis of the gene cluster putatively involved in the utilization of the sugar alcohol sorbitol in certain B. breve chromosomes. All genes are coloured coded based on their function. The percentage of similarity based on BLASTP alignment and the alcohol dehydrogenase-encoding gene targeted for the genetic insertion experiment are indicated. b) Diagram showing the growth curves of B. breve JCM 7017 wild-type and B. breve JCM 7017–1848 insertion mutant on Rogosa modified MRS (mMRS) with the addition of lactose and sorbitol 0.5% over 24 hours.