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. 2010 Jun 28;31(7):805–812. doi: 10.1038/aps.2010.65

Figure 5.

Figure 5

The effects of CD151-AAA on the proliferation, migration and tube formation of HUVECs. (A) The gene sequences of CD151 and CD151-AAA. The CD151 mutation (CD151-AAA) was undergone as follows: change the motif of QRD194–196 to AAA194–196. (B) Western blot analysis demonstrated there was no significant difference of CD151 protein expression between the CD151 group and the CD151-AAA group. (C) The effect of CD151-AAA on the phosphorylation of ERK. CD151 promoted the phosphorylation of ERK, but the CD151-AAA mutant abrogated this effect. (D) MTT assay showed that the CD151-induced proliferation of HUVECs was impaired in the CD151-AAA group. (E) Transwell Boyden chamber assays demonstrated the promoted migration of HUVECs was impaired in the CD151-AAA group. (F) and (G) The rAAV-CD151-AAA transfection abrogated the tube formation on matrigel. (F) Representative photomicrographs of tube formation. (G) Quantitative analysis of HUVECs tube formation. bP<0.05 vs the GFP group. dP>0.05, eP<0.05 vs the CD151 group. n=3. Mean±SEM.