Figure 1.
IL-27 expression during infection promotes mycobacterial growth. (A) Macrophages were infected with BCG for 48 h. Quantitative analysis of p28 and EBI3 is presented as the mean log2 change in gene expression of duplicate samples ± standard error for a representative experiment of three. Values were normalized to the mean expression of GAPDH within a sample group and expressed relative to that of medium alone. (B) Macrophages restrict the intracellular growth of BCG. Macrophages were treated with IL-12, sIL-27R, or their combination for 6 h prior to BCG infection for an additional 6, 24, 48, or 72 h. Data is represented as the mean CFUs recovered from infected macrophages ± standard error for two combined experiments. A student’s t test was used to establish statistical significance in the 95% confidence interval between individual sample groups as indicated; *indicates that IL-12 and sIL-27R is significantly different from medium alone (MED). **indicates that IL-12 and sIL-27R is significantly different from all other treatments.