Figure 2.

Treatment with IL-12 and sIL-27R induced phagosomal acidification in BCG-infected macrophages. Macrophages were treated with IL-12, sIL-27R, or their combination for 6 h prior to infection with SYTO-9® -stained BCG for 48 h. Lysotracker (100 nM) was added during the last hour of infection. (A) The images shown are from an individual experiment representative of three independent experiments (scale bar = 10 μm). (B) The percent association was calculated based on Pearson’s coefficient as described in the Methods section. These data are the combined results from three independent experiments ± standard error. A student’s t test was used to establish statistical significance in the 95% confidence interval between individual sample groups as indicated.