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. 2014 Mar 11;12:16. doi: 10.1186/1478-811X-12-16

Figure 5.

Figure 5

Treatment with IL-12 and sIL-27R increases association and maturation of cathepsin D during mycobacterial infection. Macrophages were treated with IL-12, sIL-27R, or their combination for 6 h and then infected with SYTO-61® -stained BCG for 48 h. (A) Samples were subsequently fixed with 4% PFA, permeabilized, and stained with anti-cathepsin D antibody (green). Representative images from three experiments are shown. Arrows indicate association between BCG (red) and cathepsin D (green). (B) The percent association was calculated as described in the Methods section. These data are representative results from three independent experiments. A student’s t test was used to establish statistical significance in the 95% confidence interval between individual sample groups as indicated. (C) Macrophage cultures were lysed to collect whole-cell lysates for immunoblot analysis. Cathepsin D or actin was labeled as described in the Methods section. Pro; procathepsin D (52 kDa), Pre; Precathepsin D (48 kDa), mature; mature cathepsin D (approximately 24 kDa). An image representative of two experiments is shown.