Figure 3.

Relative vg RNA expression levels under JH treatment in different social contexts. Four-day-old workers were introduced into either peer or established groups (9 and 5 workers in each category in peer and established groups, respectively) and were assigned to one of three treatments: (1) 100 ug JH-III diluted in 5 ul DMF applied to the thorax before introduction; (2) 5 μl DMF; and (3) untreated control groups. All workers were callow when introduced and were kept for 4 days. Groups were composed of full sisters originating from the same colony. Each group was taken from a different colony. Data are presented as means ± SE. Different letters above the columns represent statistical differences at α = 0.05.