Table 3.
Behavior and ovarian activation in workers introduced to peer and experienced groups
| |
Peer groups |
Experienced groups |
P value |
||||
|---|---|---|---|---|---|---|---|
| Control | DMF | JH | Control | DMF | JH | ||
| Total HDT per group |
70.8±10 |
48.9±15.1 |
52.1±16.8 |
50.75±12.2 |
54.2±12.1 |
37.3±7.3 |
p=0.56; p=0.341 |
| n=10 |
n=10 |
n=7 |
n=8 |
n=10 |
n=9 |
||
| HDT performed by IW |
36.9±13.6 |
30.6±12.1 |
35.7±15 |
14.2±3.5 |
6.3±2.3 |
4.5±1.1 |
p=0.97; p=0.022 |
| n=10 |
n=10 |
n=7 |
n=8 |
n=10 |
n=9 |
||
| HDT received by IW |
32.4±8.2 |
33.7±7.1 |
20.3±6.8 |
40.5±3.5 |
42.1±6.9 |
42±5.2 |
p=0.61; p=0.293 |
| n=10 |
n=10 |
n=7 |
n=8 |
n=10 |
n=9 |
||
| Percentage of HDT in which IW was involved |
50.6±10.9% |
35.8±15.8% |
32.1±13.4% |
26.25±6.1% |
26.9±7.4% |
17.4±3.9% |
P=0.84; p=0.0264 |
| n=10 |
n=10 |
n=7 |
n=8 |
n=10 |
n=9 |
||
| Ovarian activation of IW (4 days) |
0.14±0.01 |
0.12±0.01 |
0.17±0.01 |
0.17±0.02 |
0.16±0.02 |
0.13±0.02 |
p=0.13; p=0.685 |
| n=29 |
n=28 |
n=26 |
n=8 |
n=10 |
n=9 |
||
| Ovarian activation of IW (7 days) | 2.24±0.2 |
- |
- |
1.47±0.23 |
- |
- |
t-test: t=2.46, p=0.01 |
| (n=15) | (n=16) | ||||||
The introduced worker was assigned to one of three treatments: (1) 100ug JH-III diluted in 5 ul DMF applied to the thorax before introduction (2) 5ul DMF (3) untreated control. All workers were callow when introduced. Groups were composed of full sisters originated from the same colony (20 colonies in total) Statistical comparison was done using a nested ANOVA test. ‘IW’ refers to the introduced worker. HDT refers to the sum of humming, darting and attack behaviors received or performed by the introduced worker
1Nested ANOVA, treatment is nested in group-type: f(4,48) = 0.7, p = 0.56, For group-type only: f(1,48) = 0.91, p = 0.34.
2Nested ANOVA, treatment is nested in group-type: f(4,48) = 0.12, p = 0.97; For group-type only: f(1,48) = 5.4, p = 0.02.
3Nested ANOVA, treatment is nested in group-type: f(4,48) = 0.67, p = 0.61; For group-type only: f(1,48) = 1.14, p = 0.29.
4Nested ANOVA, treatment is nested in group-type: f(4,48) = 0.35, p = 0.84; For group-type only: f(1,48) = 5.27, p = 0.026. Data was transformed using arcsin.
5Nested ANOVA, treatment is nested in group-type: f(4,77) = 1.83, p = 0.13; For group-type only: f(1,77) = 0.16, p = 0.68.