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. 2010 Jun 4;1(6):273–276. doi: 10.1021/ml1000454

Figure 3.

Figure 3

Identification of a 75 kDa nuclear protein that covalently interacts with GO-Y086. (A) Cell lysates of GO-Y086- or GO-Y088-treated cells were incubated with avidin resin. The resin-bound proteins were analyzed by 8% SDS−PAGE and probed with antibiotin antibody. (B) CBB staining of the membrane used for the Western blot above. (C) Proteins in GO-Y086-treated cells were fractionated in a stepwise manner using ProteoExtract. A GO-Y086-bound protein in each sample was analyzed in the same manner as described in panel A. (D) Western blot analysis of the GO-Y086-bound protein using anti-KSRP antibody.