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. 2014 Jan;99(1):76–84. doi: 10.3324/haematol.2012.082768

Figure 7.

Figure 7.

Effect of the activated δ-globin gene on ineffective erythropoiesis and organ iron content in animals with a β-thalassemia intermedia phenotype (Hbbth3/+ mice) at 2.5 months of age. (A) Erythroid populations in splenic cell suspensions measured by classical CD71 and Ter119 co-staining showing the percentage of double-positive cells and single-positive Ter119 cells. FACS profiles from four representative mice: wt mice, Hbbth3/+ CACCCδ-LCR homozygous, Hbbth3/+ CACCCδ-LCR heterozygous and Hbbth3/+ transgenic lines. (B) Amount of ineffective erythropoiesis estimated in the spleen and in the bone marrow based on the ratio between early precursor (Ter119+/CD71+) and more mature erythroid cells (Ter119+/CD71−) in wt mice, Hbbth3/+CACCCδ-LCR homozygous, Hbbth3/+CACCCδ-LCR heterozygous and Hbbth3/+ transgenic lines. (C) Spleen weights normalized to body weight of wt, Hbbth3/+CACCCδ-LCR homozygous, Hbbth3/+CACCCδ-LCR heterozygous and Hbbth3/+ mice lines. (D) Spleen size observed in three representative mice: δ1) wt mouse; 2) Hbbth3/+CACCCδ-LCR homozygous and 3) Hbbth3/+ transgenic lines. (E) Total iron (mg) content of the spleen, liver and heart as measured by non-heme iron analysis in wt mice, Hbbth3/+CACCCδ-LCR homozygous, Hbbth3/+CACCCδ-LCR heterozygous and Hbbth3/+ mice transgenic lines. *P<0.05; **P<0.01; ***P<0.001 according to the t-test. Data are presented as mean ± SD. N indicates the number of mice.