Table 1. 5-HT_1AR Affinity and Fluorescence Values for Compounds 1−12.

^aThe values are the means ± SEMs from two to four independent experiments performed in triplicate.

^bFluorescence values were determined at 10 μM in binding assay buffer (50 mM Tris-HCl and 0.5 mM MgSO₄, pH 7.4) at 25 °C.

^cWavelengths for the maximal absorption and emission. The excitation slit was 2.5 nm in all cases.

^dThe Φ_f values were determined in binding assay buffer at 25 °C.

^eValue from ref (10).

^fn.d., not determined.

^gValue from ref (11).