Table 1. Thermodynamic Parameters of CaM–Fas DD Binding Determined by ITC at 37 °C and pH 5.

	Ka (M–1)a	ΔG°a (kcal/mol)	ΔH°a (kcal/mol)	–TΔS°a (kcal/mol)	n
CaM–Fas DD WT	(1.79 ± 0.20) × 106	–8.87 ± 0.07	6.00 ± 0.09	–14.87 ± 0.12	1.03 ± 0.01
CaM–Fas DD V254N	(0.88 ± 0.14) × 106	–8.43 ± 0.10	7.40 ± 0.27	–15.84 ± 0.28	1.09 ± 0.02

^aDifferences are statistically significant between CaM–Fas DD WT and CaM–Fas DD V254N mutant complexes (Student’s t test; p < 0.05).