Figure 9.

H₂O₂-induced cell apoptosis assay by flow cytometry, utilizing Annexin V vs propidium iodide (PI) staining. Cells in region R3 represent living cells (Annexin V-negative, PI-negative). Cells in region R2 represent late apoptotic cells (Annexin V-positive, PI-positive). Cells in region R1 represent early apoptotic cells (Annexin V-positive, PI-negative). Cells in region R4 represent damaged membranes only: (A) H₂O₂ (150 μM), (B) mini-αA (20 μg/mL) with H₂O₂, and (C) CP1 (20 μg/mL) with H₂O₂. (D) Average of three independent experiments.