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. 2010 Sep 28;2(1):48–55. doi: 10.1111/j.2040-1124.2010.00065.x

Figure 4.

image

 Knockdown of ATG5 gene in rIAPP‐ or hIAPP‐transfected COS‐1 cells. (a) ATG5 protein expression in COS‐1 cells co‐transfected with hIAPP and either ATG5 siRNA (Si ATG5) or non‐silencing RNA (Non Si) was analyzed by western blot with anti‐ATG5 antibodies 48 h after transfection (n = 5). (b) Cell viability 96 h after co‐transfection with each plasmid and siRNA (n = 24). (c) Western bolt analysis of light chain 3B (LC3) 48 h after co‐transfection with siRNA and hIAPP plasmid (n = 3). All values represent the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 for the differences between the indicated groups. hIAPP, human islet amyloid polypeptide; rIAPP, rat islet amyloid polypeptide.