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. 2014 May;99(5):836–847. doi: 10.3324/haematol.2013.087536

Figure 6.

Figure 6.

AML cells induce impairments in NK effector function in vitro. (A) Effect of AML blast co-culture on NK cell CD107a degranulation, TNF-α and IFN-γ production against K562. (B) Representative FACS plots showing the effect of co-incubating AML blasts from 2 different patients on healthy donor NK cell cytotoxicity and effector function are presented. After 24 h of healthy donor NK co-culture with primary AML blasts (ratio of 10:1) + IL-2 (200 iU/mL), the effector function of NK cells was assessed against K562 leukemia targets (ratio 1:1). Plots are gated on CD56+CD3− NK cells. (C) A dose effect with increasing concentrations of blasts exhibiting greater inhibition of healthy NK function.