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. Author manuscript; available in PMC: 2014 May 2.
Published in final edited form as: Cell Rep. 2013 Aug 29;4(5):870–878. doi: 10.1016/j.celrep.2013.07.041

Figure 1. Acidic residues in the hinge region of RAG2 regulate repair pathway choice.

Figure 1

(A) RAG2 schematic. Quantification of FACS data from three separate experiments for aNHEJ in CHO-K1 cells measuring mutations to known regulatory regions (B), truncation mutations (C), internal deletions (D), and neutralizing mutations (E,F). (G) XRCC4-deficient CHO cells were tested the ability to bypass cNHEJ defects using CJGFP and SJGFP (H) Recombination on the chromosomal substrate pMX-INV in SCID-MEFs with the indicated RAG2 mutations. Values significantly different than wild-type RAG1 and RAG2 are marked with an asterisk (Student’s t-test, unpaired, two tailed, equal variance). Means from three independent experiments are plotted; error bars reflect the standard error of the mean. See also Figure S1.