Skip to main content
. 2014 Apr 24;142(2):248–257. doi: 10.1111/imm.12248

Figure 4.

Figure 4

CD8 T clone recognition and termination of Chlamydia muridarum replication in epithelial cells. (a) C57ep.1 epithelial cells, untreated (a) or treated with IFN-γ (10 ng/ml for 10 hr) (b), were infected with three inclusion forming units (IFU) C. muridarum per cell. After 4 hr the inocula were removed, monolayers were washed then T cells were added. Wells were harvested 32 hr after infection; recovered IFU were quantified on McCoy monolayers. Top panels of (a) and (b) = experiment #1; bottom panels = experiment #2. For IFU values below 100 000, actual recovered IFU are shown immediately above the data bars. (c) Supernatants in experiments #1 and #2 were collected immediately before harvesting monolayers; interferon-γ (IFN-γ) levels were determined by ELISA. Aggregate data from experiments 1 and 2. **P < 0·005; ***P < 0·0005.