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. 2014 Mar 12;15(1):188. doi: 10.1186/1471-2164-15-188

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© Melicher et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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BLAST strategy to identify unique transcripts. Identification of unique transcripts in each individual assembly was performed by reserving contigs from one assembly and pooling all contigs from the remaining assemblies. The contigs from the single assembly were aligned to the pooled contigs. Contigs that fail to align were considered unique to that single assembly. The unique contigs were annotated by aligning to the D. melanogaster transcriptome.