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. 2014 Mar 12;15(1):188. doi: 10.1186/1471-2164-15-188

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© Melicher et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Extension of extradenticle sequence by meta-assembly. Contigs generated by multiple k-mer lengths were consolidated by meta-assembly to recover the entire coding sequence of the gene extradenticle from sequence fragments. Contigs from individual assemblies of multiple k-mer lengths are shown in alignment to the meta-assembly and the Drosophila transcript. The k-mer length 31 contigs were not included in the meta-assembly and show a reduction in coverage compared to other assemblies. Assemblies with shorter k-mer lengths also show a reduction in coverage but are not shown due to excessive fragmentation which results in a large number of short contigs that cannot be confidently aligned. The extended transcript aligns to the full length of the Drosophila reference sequence with 83% nucleotide sequence conservation.