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. 2014 May 2;9(5):e95441. doi: 10.1371/journal.pone.0095441

Figure 1. L. rhamnosus and B. breve alter T cell differentiation in human PBMCs.

Figure 1

PBMCs were stimulated with anti-CD3 alone (white bars), with a combination of anti-CD3 and L. rhamnosus (grey bar) or a combination of anti-CD3 and B. breve (black bar) for 48 hours or 7 days. A–D) The percentages of Th2 (GATA3+Tbet-), Th17 (RORγ+FOXP3-), Treg (RORγ-FOXP3+) or Th1 (GATA-Tbet+) cells within the activated T cells (CD4+CD69+) in the PBMCs were determined after 48 hours of incubation. Percentages within activated CD4+CD69+ T cell population are shown. E–H) The percentages of cytokines (IL10, IL17, IL4 or IFNγ) producing CD4+ T cells in the PBMCs were determined after 7 days of incubation. Percentages within CD4+ T cell population are shown. The Results are expressed as mean + SEM, n = 3, * p<0.05.