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. 2014 May 2;9(5):e95441. doi: 10.1371/journal.pone.0095441

Figure 2. B. breve, but not L. rhamnosus, ameliorates DSS-induced colitis.

Figure 2

C57BL/6 mice with or without probiotics treatment received either normal drinking water or drinking water with DSS for 5 days. A) The fecal condition was calculated on day 0, day 3 and day 5 after DSS treatment. On day 6, the mice were sacrificed and B) the colon length of each mouse was measured. Results are expressed as mean ± SEM, n = 6 mice per group, pooled from two independent experiments. Colons were collected and examined for histological score as described in materials and methods. C) The histological scoring graph and D) representative H&E staining photos are shown. Results are expressed as mean + SEM, n = 3 mice per group, pooled from two independent experiments. E) The presence of Ly-6B+ cells was visualized in the proximal (p) and distal (d) colons using immunohistochemistry. The pictures are representative of 3 separate mice per group obtained from two experiments. F) The concentration of MPO was measured in colon homogenates of each group. Results are expressed as mean + SEM, n = 4 mice per group, pooled from two independent experiments. * p<0.05; ** p<0.01.