Table 4. The thermodynamic constants obtained by isothermal titration calorimetry for the association of nucleosides with Ago2PAZ domain.

substrate	T	Ka	K d	ΔH	ΔS
	°C	M−1*104	(µM)	(cal/mol)	(cal/mol)
UMP	8	6.3±0.4	15.8±0.5	−1230±66	21±1
	15	1.6±0.2	62.5±1.5	−238±15	18.4±1.1
	30	1.3±0.1	77±2.2	−89±5	12±1.2
GMP	8	4.9±0.2	20±1	−251±20	20.6±1
	15	4±0.45	25±1.2	−173±10	21.4±1.1
	30	1.9±0.1	52±4	−84±0.5	12.5±0.8

The one set of sites binding model is used for fitting the data. (n = 3).