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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Mol Microbiol. 2014 Feb 28;92(1):28–46. doi: 10.1111/mmi.12538

Figure 6. Reduced Mu replication in the presence of truncated IF2 at 0 or low levels of ppGpp.

Figure 6

A. Phage Mucts62 plating efficiency on priA300 strains expressing IF2-1 or IF2-2/3 from plasmid vectors pSPCinfB(del2/3 or del1). Strains (from left to right): GTN1800, GTN1757, GTN1759, GTN1860, GTN1858, GTN1856, GTN1675, GTN1957.

B. Levels of Mu replication in vitro with IF2-2 and 1 mM GTP, GDP, and ppGpp. The amount of replication obtained without the added 1 mM guanine nucleotides was set to 100 units (approximately 25% of total Mu DNA replicated).

C. Effect of GTP, GDP, and ppGpp on the binding of S-IF2-2 to Mu DNA in vitro. The level of binding when no nucleotides were present is set to 100 units.