(A) Photomicrographs of the hippocampal sections obtained from 1, 2, 5, and
12 m.o. mouse demonstrate the decline in the number of NBs
(PSA-NCAM+) and the number of apoptotic profiles, identified by
nuclear morphology (pyknosis) and phagocytosis by microglia (Iba1+),
pointed by arrows. Numbered insights represent higher magnification of the
identified apoptotic cells.
(B) Quantification of the number of apoptotic cells in the SGZ during
adulthood. The number of SGZ apoptotic cells decreases sharply after 1 month
of age.
(C) Quantification of the Ph-index. The microglial phagocytic efficiency
remains constant throughout adulthood.
Scale bars; low magnification, 50μm (z=16.24μm); high
magnification, 10μm. Bars represent mean ± SEM.