Table 2. PUFA composition of total cellular lipids from the CHO cells transfected with EGFP, F2F1 or F2F1+D4.
Mol % of fatty acids | EGFP | F2F1 | F2F1+D4 |
14∶0 | 4.2±0.83 | 3.8±0.81 | 3.2±0.78 |
16∶0 | 0.7±0.03 | 0.8±0.04 | 2.1±0.11 |
18∶0 | 18.8±2.12 | 19.6±2.33 | 18.2±1.98 |
18∶1n-9 | 20.3±1.18 | 20.3±1.12 | 20.7±1.27 |
18∶1n-7 | 10.9±0.91 | 10.3±0.95 | 8.2±0.78 |
n-6 PUFA | |||
18∶2n-6 | 16.1±1.23a | 12.6±1.07b | 12.3±0.92b |
20∶3n-6 | 0.9±0.07b | 1.7±0.21a | 1.9±0.20a |
20∶4n-6 | 5.6±0.88b | 7.1±0.69a | 6.8±0.70a |
22∶4n-6 | 1.5±0.09 | 1.4±0.14 | 1.5±0.21 |
Total | 24.0±1.91 | 22.7±1.76 | 22.5±1.69 |
n-3 PUFA | |||
18∶3n-3 | 6.9±0.84a | 4.0±0.37b | 3.4±0.29b |
20∶3n-3 | 3.0±0.23a | 2.1±0.31b | 1.8±0.13b |
20∶4n-3 | 1.3±0.12a | 0.9±0.13b | 0.9±0.09b |
20∶5n-3 | 0.6±0.08c | 2.6±0.29a | 1.8±0.15b |
22∶5n-3 | 3.9±0.71b | 7.2±0.99a | 4.2±0.73b |
22∶6n-3 | 4.7±0.48b | 5.3±0.66b | 12.5±0.85a |
Total | 20.4±1.30b | 22.0±1.07b | 24.6±1.51a |
Values are means of three measurements. Values for each fatty acid with different superscripts differ significantly (P<0.05) between control (EGFP), sN3 and N3D4.