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. 2014 May 2;9(5):e96390. doi: 10.1371/journal.pone.0096390

Figure 1. FCF binding induces a shift in the thermal denaturation profile of SEPT2.

Figure 1

(A) Curve plots show the fluorescence intensity (relative fluorescence units; RFU) of SYPRO ORANGE as a function of temperature for purified recombinant SEPT2 protein in the presence of DMSO carrier and increasing concentrations of FCF. (B) The negative first derivative of the SYPRO ORANGE fluorescence intensity (RFU) was plotted against temperature. The melting curves illustrate the transition temperatures during the denaturation of SEPT2. Insert shows the median melting temperature of SEPT2 from three independent experiments in the presence of increasing FCF concentrations. Error bars represent the highest and lowest values obtained from three independent experiments.