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. 2014 May 2;9(5):e96326. doi: 10.1371/journal.pone.0096326

Figure 3. Overexpressing HA-cpa suppresses the apical F-actin accumulation of cpbL262R-expressing wing discs, whereas HA-cpaΔABD expression has the opposite effect.

Figure 3

(A–A″ to E–E″) standard confocal sections of the apical cell membrane of third instar wing imaginal discs with dorsal sides up and posterior sides to the left, expressing (A–A′) one copy of UAS-mCD8-GFP (green in A) or (B–B″) UAS-cpb7 and one copy of UAS-mCD8-GFP (green in B) or (C–C″) UAS-cpbL262R and two copies of UAS-mCD8-GFP (green in C) or (D–D″) UAS-cpbL262R, UAS-HA-cpa89E and one copy of UAS-mCD8-GFP (green in D) or (E–E″) UAS-cpbL262R, UAS-HA-cpaΔABD and one copy of UAS-mCD8-GFP (green in E) under hh-Gal4 control. Discs are stained with Phalloidin (white) to mark F-actin and (B–B″ to E–E″) anti-Cpb (cyan blue). The yellow lines outline the anterior-posterior compartment boundary. The scale bars represent 30 µm. (F) Mean intensity of the ratio of Phalloidin signal between posterior and anterior wing compartments of hh-Gal4 driving two copies of UAS-mCD8-GFP (lane 1) or UAS-HA-cpa89E, UAS-cpb7 and one copy of UAS-mCD8-GFP (lane 2) or UAS-HA-cpaΔABD, UAS-cpb7 and one copy of UAS-mCD8-GFP (lane 3) or UAS-cpb7 and one copy of UAS-mCD8-GFP (lane 4) or UAS-cpbL262R and two copies of UAS-mCD8-GFP (lane 5) or UAS-cpbL262R, UAS-HA-cpa89E and one copy of UAS-mCD8-GFP (lane 6) or UAS-cpbL262R, UAS-HA-cpaΔABD and one copy of UAS-mCD8-GFP (lane 7). The mean for lane 1 is 0.922 (n = 12) for lane 2 is 0.775 (n = 8), for lane 3 is 1.435 (n = 10), for lane 4 is 0.977 (n = 10), for lane 5 is 1.175 (n = 16), for lane 6 is.0.937 (n = 14), for 7 is 2.348 (n = 6). Error bars indicate s.e.m.. *** indicate P<0.0001.