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. 2014 May 2;9(5):e96326. doi: 10.1371/journal.pone.0096326

Figure 4. Overexpressing full length HA-cpa and cpb prevents wing growth, while ectopic expression of HA-cpaΔABD and/or cpbL262R has the opposite effect.

Figure 4

(A, B, C, D and E) merge between adult wings expressing in green UAS-mCD8GFP under nub-Gal4 control and in magenta (A) UAS-HA-cpa89E and UAS-cpb7 or (B) UAS-HA-cpaΔABD and UAS-cpb7 or (C) UAS-cpbL262R and one copy of UAS-mCD8-GFP or (D) UAS-cpbL262R and UAS-HA-cpa89E or (E) UAS-cpbL262R and UAS-HA-cpaΔABD under nub-Gal4 control. (A′, B′, C′ D′ and E′) magnification of hairs on adult wings for the genotypes shown in A, B, C, D and E. (F) quantification of relative wing size normalized to nub>GFP control for nub-Gal4 driving UAS-mCD8-GFP (lane 1) or UAS-HA-cpa89E and UAS-cpb7 (lane 2) or UAS-HA-cpaΔABD and UAS-cpb7 (lane 3) or UAS-cpbL262R and one copy of UAS-mCD8-GFP (lane 4) or UAS-cpbL262R and UAS-HA-cpa89E (lane 5) or UAS-cpbL262R and UAS-HA-cpaΔABD (lane 6). The mean for lane 1 is 1(n = 32), for lane 2 is 0.9702 (n = 12), for lane 3 is 1.119 (n = 13), for lane 4 is 1.061 (n = 24), for lane 5 is 1.015 (n = 13), for lane 6 is 1.051 (n = 13). Error bars indicate s.e.m.. P<0.015 for comparison of lanes 1 and 2. P<0.0001 for comparison of lanes 1 and 3 or 4 or 6 and for comparison of lane 4 and 5.