Table 2. Fold-change of tissue fatty acid amide levels following treatment with URB597 or WIN 55,212-2 relative to vehicle treatment.

Seven days

AEA

OEA

PEA

Rats

Treatment

Brain

Rostral

Epicenter

Caudal

Lumbar

Brain

Rostral

Epicenter

Caudal

Lumbar

Brain

Rostral

Epicenter

Caudal

Lumbar

SCI

Vehicle

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

SCI

URB

1.6 (*)

4.3 (**)

0.9 (n.s.)

6.1 (*)

2.9 (*)

5.3 (*)

4.2 (***)

1.2 (n.s.)

8.2 (*)

2.8 (**)

6.5 (***)

3.6 (***)

1.2 (n.s.)

7.5 (*)

3.5 (*)

SCI

WIN

0.5 (n.s.)

1.6 (n.s.)

0.2 (n.s)

1.3 (n.s.)

0.9 (n.s.)

0.4 (n.s.)

1.3 (n.s.)

0.3 (n.s.)

1.2 (n.s.)

0.9 (n.s.)

0.6 (n.s.)

1.3 (n.s.)

0.3 (n.s.)

1.3 (n.s.)

1.3 (n.s.)

One day

N.I.

Vehicle

1

—

1

—

1

1

—

1

—

1

1

—

1

—

1

N.I.

URB (2 h)

2.7 (*)

—

6.5 (***)

—

2.5 (***)

5.1 (***)

—

6.3 (***)

—

2.3 (***)

5.7 (***)

—

3.8 (***)

—

2.3 (***)

N.I.

URB

1.1 (n.s.)

—

3.8 (***)

—

3.0 (***)

2.5 (***)

—

3.5 (***)

—

2.2 (***)

3.6 (***)

—

2.6 (***)

—

2.0 (***)

N.I.

WIN

0.6 (n.s.)

—

0.8 (n.s.)

—

1.6 (n.s)

0.6 (n.s.)

—

0.8 (n.s.)

—

1 (n.s.)

1 (n.s.)

—

0.8 (n.s.)

—

1 (n.s.)

Values are fold-changes compared to vehicle treatment levels in spinal cord injured (SCI) and non-injured (N.I.) rats. On the seventh day of treatment, tissues were collected 4 hours post-administration with either vehicle, URB597 (URB, 3 mg/kg, i.p.) or WIN 55,212-2 (WIN, 3 mg/kg, s.c.) from SCI rats. Non-injured rats were treated once and tissues collected four hours post-treatment. In one set of non-injured rats (n = 5), tissues were collected two hours (2 h) post-treatment with URB597.

“Epicenter” refers to thoracic spinal cord tissue either from the site of injury from SCI rats or comparable spinal cord tissue from non-injured rats.

n.s., not significant.

*p<0.05, **p<0.01, ***p<0.001 vs. vehicle-treated (Student's t-test).