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. 2014 May 2;9(5):e96565. doi: 10.1371/journal.pone.0096565

Figure 1. T cells infiltrate PDA regardless of GM-CSF expression.

Figure 1

A-C) IHC of normal adjacent pancreas, well-differentiated PDA, and poorly differentiated PDA showing dual staining of T cells (CD3+ in brown, arrows) and myeloid cells (CD11b+in purple, arrowheads). Open arrowhead indicates a normal duct. Photomicrographs taken with a 20x objective. D) Quantification of CD3+ and CD11b+ cells in PDA (p = 0.0009). E) Correlation between density of CD3+ and CD11b+ cells in each tumor (r = 0.51; p = 0.0008). F-H) Representative photomicrographs (40x) showing GM-CSF expression in normal pancreas and PDA. I) Distribution of myeloid cells and T cells stratified based upon chromogen intensity for GM-CSF. At least five 40x fields were analyzed per specimen. Scale bars = 25 µm.