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. 2014 May 2;9(5):e96608. doi: 10.1371/journal.pone.0096608

Figure 1. TLR-1/2 activation effects on expression of β7, αVβ3 and α4 integrins in HMCLs.

Figure 1

HMCLs were stimulated with Pam3CSK4 for 24 hours and then applied to FACS analysis for expression of integrin molecules as explained in materials and methods. Pam3CSK4 downregulated β7 expression in Fravel, OPM-1, OPM-2, and NCI-H929 in a dose-dependent manner. No change in β7 expression was observed in any other cell line. Pam3CSK4 up-regulated αVβ3 and α4 expression dose-dependently in all HMCLs except RPMI-8226. The results are the statistical analyses of data in 3 separate experiments expressed as mean ± SEM, *P<0.05, **P<0.01, ***P<0.001. (Ust: Unstimulated).