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. 2014 May 2;9(5):e96465. doi: 10.1371/journal.pone.0096465

Figure 6. Q-PCR analysis of the expression of hypoxia inducible factor 1alpha (HIF-1α), Erythropoietin (EPO) and vascular endothelial growth factor (VEGF).

Figure 6

Q-PCR analysis was performed for midbrain and forebrain cultures differentiated for 10 days in vitro (DIV) (sequential addition of FGF8, Shh, GDNF, and Forskolin) at either high (20%) or low (3%) oxygen tension. Analysis of HIF-1alpha mRNA revealed no significant difference between midbrain or forebrain cultures differentiated at high or low oxygen tension (A). Analysis of EPO mRNA revealed a strong tendency for a higher level in midbrain and forebrain cultures differentiated at low as compared to high oxygen (B). A significantly higher level of VEGF mRNA was found in midbrain cultures differentiated at low as compared to high oxygen, whereas no significant difference was found between forbrain cultures grown at low as compared to high oxygen tension however, there was a tendency for higher expression of VEGF in the forebrain cultures differentiated at low oxygen (C). Data are expressed as means±SEM (12 replicates/group, two independent experiments, *P<0.05).